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eea1 ab alexa fluor 546  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology eea1 ab alexa fluor 546
    BBAs delayed EV71 entry through the endosomal compartment. ( A ) HeLa cells were inoculated with EV71 stock at an MOI of 300 at 4°C for 1 h for adsorption. Afterward, the cells were treated with TET or CEP at 10 µM for 90 min before being fixed and permeabilized. Puncta signals (green) representing EV71 virions were detected using a rabbit anti-VP2 Ab, followed by detection with a secondary Ab conjugated to Alexa Fluor 488. DAPI was used to stain the nucleus (blue). Scale bar represents 20 µM. ( B ) The number of puncta surrounding each cell was calculated based on images from five fields. ( C ) Fluorescence signals were detected at the indicated time points after adsorption with EV71. Cells were co-stained with Ab against viral VP2 and ( D ) <t>EEA1</t> (indicating early endosomes) or ( E ) LAMP1 (indicating lysosomes). The portion of VP2 colocalized with EEA1 or LAMP1 is shown as the average ± SEM for more than 200 cells. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05.
    Eea1 Ab Alexa Fluor 546, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eea1+ab+alexa+fluor+546/EEA1+Antibody/pmc12691679-107-6-14
    Average 96 stars, based on 720 article reviews
    eea1 ab alexa fluor 546 - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Cepharanthine inhibits enterovirus entry by endolysosomal deacidification and exhibits protective activity in vivo"

    Article Title: Cepharanthine inhibits enterovirus entry by endolysosomal deacidification and exhibits protective activity in vivo

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/aac.00764-25

    BBAs delayed EV71 entry through the endosomal compartment. ( A ) HeLa cells were inoculated with EV71 stock at an MOI of 300 at 4°C for 1 h for adsorption. Afterward, the cells were treated with TET or CEP at 10 µM for 90 min before being fixed and permeabilized. Puncta signals (green) representing EV71 virions were detected using a rabbit anti-VP2 Ab, followed by detection with a secondary Ab conjugated to Alexa Fluor 488. DAPI was used to stain the nucleus (blue). Scale bar represents 20 µM. ( B ) The number of puncta surrounding each cell was calculated based on images from five fields. ( C ) Fluorescence signals were detected at the indicated time points after adsorption with EV71. Cells were co-stained with Ab against viral VP2 and ( D ) EEA1 (indicating early endosomes) or ( E ) LAMP1 (indicating lysosomes). The portion of VP2 colocalized with EEA1 or LAMP1 is shown as the average ± SEM for more than 200 cells. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05.
    Figure Legend Snippet: BBAs delayed EV71 entry through the endosomal compartment. ( A ) HeLa cells were inoculated with EV71 stock at an MOI of 300 at 4°C for 1 h for adsorption. Afterward, the cells were treated with TET or CEP at 10 µM for 90 min before being fixed and permeabilized. Puncta signals (green) representing EV71 virions were detected using a rabbit anti-VP2 Ab, followed by detection with a secondary Ab conjugated to Alexa Fluor 488. DAPI was used to stain the nucleus (blue). Scale bar represents 20 µM. ( B ) The number of puncta surrounding each cell was calculated based on images from five fields. ( C ) Fluorescence signals were detected at the indicated time points after adsorption with EV71. Cells were co-stained with Ab against viral VP2 and ( D ) EEA1 (indicating early endosomes) or ( E ) LAMP1 (indicating lysosomes). The portion of VP2 colocalized with EEA1 or LAMP1 is shown as the average ± SEM for more than 200 cells. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05.

    Techniques Used: Adsorption, Staining, Fluorescence

    Related Articles

    Adsorption:

    Article Title: Cepharanthine inhibits enterovirus entry by endolysosomal deacidification and exhibits protective activity in vivo
    Article Snippet: To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.. Additionally, cells were co-stained with either EEA1 Ab Alexa Fluor 546 (G-4, 1:500, sc-137130, Santa Cruz) or LAMP1 Ab Alexa Fluor 546 (H4A3, 1:500, sc-20011, Santa Cruz).. The cells were also stained with DAPI to indicate the location of the nucleus.The cells were also stained with DAPI to indicate the location of the nucleus.

    Article Title: Cepharanthine inhibits enterovirus entry by endolysosomal deacidification and exhibits protective activity in vivo .
    Article Snippet: To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.. Additionally, cells were co-stained with either EEA1 Ab Alexa Fluor 546 (G-4, 1:500, sc-137130, Santa Cruz) or LAMP1 Ab Alexa Fluor 546 (H4A3, 1:500, sc-20011, Santa Cruz).. The cells were also stained with DAPI to indicate the location of the nucleus.The cells were also stained with DAPI to indicate the location of the nucleus.

    Staining:

    Article Title: Cepharanthine inhibits enterovirus entry by endolysosomal deacidification and exhibits protective activity in vivo
    Article Snippet: To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.. Additionally, cells were co-stained with either EEA1 Ab Alexa Fluor 546 (G-4, 1:500, sc-137130, Santa Cruz) or LAMP1 Ab Alexa Fluor 546 (H4A3, 1:500, sc-20011, Santa Cruz).. The cells were also stained with DAPI to indicate the location of the nucleus.The cells were also stained with DAPI to indicate the location of the nucleus.

    Article Title: Cepharanthine inhibits enterovirus entry by endolysosomal deacidification and exhibits protective activity in vivo .
    Article Snippet: To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.. Additionally, cells were co-stained with either EEA1 Ab Alexa Fluor 546 (G-4, 1:500, sc-137130, Santa Cruz) or LAMP1 Ab Alexa Fluor 546 (H4A3, 1:500, sc-20011, Santa Cruz).. The cells were also stained with DAPI to indicate the location of the nucleus.The cells were also stained with DAPI to indicate the location of the nucleus.

    Fluorescence:

    Article Title: Cepharanthine inhibits enterovirus entry by endolysosomal deacidification and exhibits protective activity in vivo
    Article Snippet: To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.. Additionally, cells were co-stained with either EEA1 Ab Alexa Fluor 546 (G-4, 1:500, sc-137130, Santa Cruz) or LAMP1 Ab Alexa Fluor 546 (H4A3, 1:500, sc-20011, Santa Cruz).. The cells were also stained with DAPI to indicate the location of the nucleus.The cells were also stained with DAPI to indicate the location of the nucleus.

    Article Title: Cepharanthine inhibits enterovirus entry by endolysosomal deacidification and exhibits protective activity in vivo .
    Article Snippet: To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.To detect the viral antigen, we used rabbit anti-EV71 VP2 polyclonal Ab (1:2,000, GTX132340, GeneTex) as the primary Ab and Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (1:500, ab150077, Abcam) as the secondary Ab.. Additionally, cells were co-stained with either EEA1 Ab Alexa Fluor 546 (G-4, 1:500, sc-137130, Santa Cruz) or LAMP1 Ab Alexa Fluor 546 (H4A3, 1:500, sc-20011, Santa Cruz).. The cells were also stained with DAPI to indicate the location of the nucleus.The cells were also stained with DAPI to indicate the location of the nucleus.



    Similar Products

    eea1 ab alexa fluor 546  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology eea1 ab alexa fluor 546
    BBAs delayed EV71 entry through the endosomal compartment. ( A ) HeLa cells were inoculated with EV71 stock at an MOI of 300 at 4°C for 1 h for adsorption. Afterward, the cells were treated with TET or CEP at 10 µM for 90 min before being fixed and permeabilized. Puncta signals (green) representing EV71 virions were detected using a rabbit anti-VP2 Ab, followed by detection with a secondary Ab conjugated to Alexa Fluor 488. DAPI was used to stain the nucleus (blue). Scale bar represents 20 µM. ( B ) The number of puncta surrounding each cell was calculated based on images from five fields. ( C ) Fluorescence signals were detected at the indicated time points after adsorption with EV71. Cells were co-stained with Ab against viral VP2 and ( D ) <t>EEA1</t> (indicating early endosomes) or ( E ) LAMP1 (indicating lysosomes). The portion of VP2 colocalized with EEA1 or LAMP1 is shown as the average ± SEM for more than 200 cells. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05.
    Eea1 Ab Alexa Fluor 546, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eea1+ab+alexa+fluor+546/EEA1+Antibody/pmc12691679-107-6-14
    Average 96 stars, based on 1 article reviews
    eea1 ab alexa fluor 546 - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    BBAs delayed EV71 entry through the endosomal compartment. ( A ) HeLa cells were inoculated with EV71 stock at an MOI of 300 at 4°C for 1 h for adsorption. Afterward, the cells were treated with TET or CEP at 10 µM for 90 min before being fixed and permeabilized. Puncta signals (green) representing EV71 virions were detected using a rabbit anti-VP2 Ab, followed by detection with a secondary Ab conjugated to Alexa Fluor 488. DAPI was used to stain the nucleus (blue). Scale bar represents 20 µM. ( B ) The number of puncta surrounding each cell was calculated based on images from five fields. ( C ) Fluorescence signals were detected at the indicated time points after adsorption with EV71. Cells were co-stained with Ab against viral VP2 and ( D ) EEA1 (indicating early endosomes) or ( E ) LAMP1 (indicating lysosomes). The portion of VP2 colocalized with EEA1 or LAMP1 is shown as the average ± SEM for more than 200 cells. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Cepharanthine inhibits enterovirus entry by endolysosomal deacidification and exhibits protective activity in vivo

    doi: 10.1128/aac.00764-25

    Figure Lengend Snippet: BBAs delayed EV71 entry through the endosomal compartment. ( A ) HeLa cells were inoculated with EV71 stock at an MOI of 300 at 4°C for 1 h for adsorption. Afterward, the cells were treated with TET or CEP at 10 µM for 90 min before being fixed and permeabilized. Puncta signals (green) representing EV71 virions were detected using a rabbit anti-VP2 Ab, followed by detection with a secondary Ab conjugated to Alexa Fluor 488. DAPI was used to stain the nucleus (blue). Scale bar represents 20 µM. ( B ) The number of puncta surrounding each cell was calculated based on images from five fields. ( C ) Fluorescence signals were detected at the indicated time points after adsorption with EV71. Cells were co-stained with Ab against viral VP2 and ( D ) EEA1 (indicating early endosomes) or ( E ) LAMP1 (indicating lysosomes). The portion of VP2 colocalized with EEA1 or LAMP1 is shown as the average ± SEM for more than 200 cells. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05.

    Article Snippet: Additionally, cells were co-stained with either EEA1 Ab Alexa Fluor 546 (G-4, 1:500, sc-137130, Santa Cruz) or LAMP1 Ab Alexa Fluor 546 (H4A3, 1:500, sc-20011, Santa Cruz).

    Techniques: Adsorption, Staining, Fluorescence